Some species of Carnivora have been thought to emit a chemical signal by placing their faeces or urine in a conspicuous manner. Although many studies suggest that animals of Carnivora select the locations of defaecation, field survey methods were biased and/or a proper statistical analysis were not applied in most studies. We investigated latrine sites with an unbiased method in a natural forest of Hokkaido. To minimize the oversight of latrine sites, three or more researchers walked the study area (153.4 ha) thoroughly in the line at 10 m intervals and observed characteristics of all latrines and latrine sites (mound, flat, and pit type topography). We also investigated the amount of available resource for latrine sites by observing characteristics of randomly selected locations in the study area. Most latrines (61, 54%) were found on mound type sites, followed by flat sites (52, 46%). No latrines were found at pit type sites. In contrast, the amount of flat type latrines (95, 84%) dominated over other types in available resources. It is therefore concluded that mound type topography was selected for latrine sites, whereas the other two types (flat and pit) were avoided.